Title
Controlled Protonation of [2Fe–2S] Leading to MitoNEET Analogues and Concurrent Cluster Modification
Document Type
Article
Publication Date
11-1-2021
Abstract
MitoNEET, a key regulatory protein in mitochondrial energy metabolism, exhibits a uniquely ligated [2Fe–2S] cluster with one histidine and three cysteines. This unique cluster has been postulated to sense the redox environment and release Fe–S cofactors under acidic pH. Reported herein is a synthetic system that shows how [2Fe–2S] clusters react with protons and rearrange their coordination geometry. The low-temperature stable, site-differentiated clusters [Fe2S2(SPh)3(CF3COO)]2– and [Fe2S2(SPh)3(py)]− have been prepared via controlled protonation below −35 °C and characterized by NMR, UV–vis, and X-ray absorption spectroscopy. Both complexes exhibit anodically shifted redox potentials compared to [Fe2S2(SPh)4]2– and convert to [Fe4S4(SPh)4]2– upon warming to room temperature. The current study provides insight into how mitoNEET releases its [2Fe–2S] in response to highly tuned acidic conditions, the chemistry of which may have further implications in Fe–S biogenesis.
Identifier
PMID: 34672568
DOI
10.1021/acs.inorgchem.1c02622
Publisher
American Chemical Society
Repository Citation
Oakley, K., Sterling, K., Shearer, J., & Kim, E. (2021). Controlled protonation of [2Fe-2S] leading to MitoNEET analogues and concurrent cluster modification. Inorganic Chemistry, 60(21), 16074-16078. https://doi.org/10.1021/acs.inorgchem.1c02622
Publication Information
Inorganic Chemistry